Cell culture flask utilizing membrane barrier

ABSTRACT

A cell culture flask (2) includes a hollow housing (4) and a gas permeable membrane disposed within the housing which divides the housing into a first chamber (50) and a second chamber (52). A retaining ring (22) secures the gas permeable membrane within the housing and forms a liquid-tight seal between the first chamber and the second chamber. A gas inlet (16) in the housing communicates with the first chamber and supplies gas thereto. A media (46) inlet in the housing communicates with the second chamber and provides cell growth media thereto.



